2019-nCoV Neutralizing Antibody Test (Colloidal Gold)
Product Detail:
Innovita® 2019-nCoV IgM/IgG Bvunzo yakagadzirirwa kucherechedzwa kwehafu-yepamusoro yekudzivirira masoja ekudzivirira chirwere kune novel coronavirus (2019-nCoV) muserum yevanhu, plasma kana sampuli dzeropa rose.
2019-nCoV inosanganisira mapuroteni mana makuru ezvimiro: S protein, E protein, M protein uye N protein.Iyo RBD dunhu reS protein inogona kusunga kune yemunhu cell surface receptor ACE2.Neutralizing antibody zvinoreva kugona kusunga nehutachiona, uyezve kuvhara pathogen kuti ipinde mumuviri kukonzera hutachiona.Kuonekwa kwekusaurura masoja ekudzivirira chirwere kunogona kushandiswa kuongorora fungidziro yehutachiona hwehutachiona.
Musimboti:
Iyo kit ndeye colloid yegoridhe immunochromatography yemakwikwi assay yekuona neutralizing masoja ekudzivirira chirwere kusvika ku2019-nCoV muserum yemunhu, plasma kana sampuli dzeropa rose.Mushure mekunge mufananidzo wacho waiswa pamuenzaniso zvakanaka, kana masoja ekudzivirira emuviri aripo mumuenzaniso, masoja ekudzivirira chirwere anozobatana negoridhe rekolloidal rakanyorwa kuti RBD antigen kugadzira immune complex, uye nzvimbo yekusaurura yeyakanzi RBD antigen inovharwa.Ipapo iyo immune complex uye yakanyorwa RBD antigen isina kusunga kune neutralizing antibody inotama ichitevedza nitrocellulose membrane.Kana vasvika kunzvimbo yekuyedza (T mutsara), iyo yakanyorwa kuti RBD antigen isina kusungirira kune inodzivirira masoja ekudzivirira chirwere ichaita neACE2 antigen yakavharwa panitrocellulose membrane uye kugadzira mutsara wepepuru-tsvuku.Kana uwandu hwemasoja ekudzivirira chirwere huchikwirira kudarika muganhu wekuona wakaderera, mutsara wepepuru-tsvuku unoreruka pane mutsara wekutonga (C line) kana pasina mutsara wepepuru-tsvuku wakaumbwa, mhedzisiro yakanaka.Kana uwandu hwemasoja ekudzivirira chirwere akadzikira pane muganhu wekuona wakaderera kana pasina masoja ekudzivirira chirwere mumuenzaniso, mutsara wepepuru-tsvuku unosviba kupfuura mutsara wekutonga, mhedzisiro yacho yakaipa.
Kunyangwe iyo sampuli ine 2019-nCoV neutralizing antibodies, kana colloidal goridhe-rakanyorwa huku IgY antibody ikatamira kumutsara wekutonga (C mutsara), inobatwa nembudzi anti-huku IgY antibody yakavharirwa pamutsetse wekutonga (C). mutsara), mutsara wepepuru-tsvuku unoumbwa.Mutsetse wekutonga (C mutsara) unoshandiswa senzira yekudzora.Mitsetse yekudzora inofanirwa kugara ichionekwa mumahwindo emhedzisiro kana maitiro ekuyedza akaitwa nemazvo uye mareagents ari kushanda sezvakarongwa.
Kuumbwa:
| IFU | 1 |
| Test cassette | 40 |
| Muenzaniso diluent | 6mL * 2 mabhodhoro |
Test Procedure:
1. Vhura homwe yealuminium foil uye buritsa kaseti yebvunzo.
2. Isa 40μL ye serum / plasma specimen kana 60μL rose reropa remuenzaniso kune muenzaniso zvakanaka.
3. Isa 40μL (2 madonhwe) emuenzaniso diluent kumuenzaniso zvakanaka.
4. Iise panzvimbo yekushisa (15 ℃ ~ 30 ℃) kwemaminetsi gumi nemashanu-20, uye uverenge chigumisiro.
Dudziro yezvabuda:
1. Positive: Kana ruvara rwemutsetse weT ruchireruka pane C kana pasina T mutsara, zvinoratidza zvakanaka pakuderedza masoja ekudzivirira chirwere.
2. Negative: Kana ruvara rwemutsara weT rwasviba pane kana kuenzana neremutsetse weC, rinoratidza zvakaipa pakuderedza masoja ekudzivirira chirwere.
3. Hazvina maturo: Kana C line yakundikana kuonekwa, pasinei nokuti mutsetse weT unoonekwa here kana kuti kwete, muedzo wacho haubvumirwi.Dzokorora bvunzo nebvunzo nyowani.
