Novel Coronavirus (2019-nCoV) Nucleic Acid Test Kit
Product Detail:
Innovita® 2019-nCoV IgM/IgG Test yakagadzirirwa kuongororwa uye epidemiological yekutarisa zvirwere zvinokonzerwa neinoveli coronavirus (2019-nCoV).ORF1ab uye N gene ye2019-nCoV inowonekwa zvakanaka kubva pahuro swabs uye alveolar lavage fluid samples inounganidzwa kubva kune vanofungidzirwa nyaya dzepneumonia, varwere vane nyaya dzinofungidzirwa, vamwe vanoda kuongororwa.
Mhedzisiro yebvunzo dzekiti iyi ndeyekuongororwa kwekiriniki chete.Zvinokurudzirwa kuita ongororo yakazara yemamiriro acho zvichienderana nemurwere kuratidzwa kwekiriniki uye mamwe ma laboratory bvunzo.
Musimboti:
Iyi kit inoshandisa imwe-nhanho reverse transcript polymerase chain reaction (RT-PCR) tekinoroji yekuona kunanga iyo novel coronavirus (2019-nCoV) ORF1ab gene, N gene, uye yemukati yemunhu referensi gene kutevedzana.Chaiyo primers uye taqman probes dzakagadzirwa munzvimbo dzakachengetedzwa.
Kuumbwa:
| Composition | 48 bvunzo / kiti |
| Reaction Mix A | 792μL×1Tube |
| Reaction Mix B | 168μL×1Tube |
| Kudzora Kwakanaka | 50μL×1Tube |
| Negative Control | 50μL × 1Tube |
Cherechedza: 1. Mabheji akasiyana e reagents haafaniri kusanganiswa.
2. Positive controls uye positive controls haifaniri kutorwa
Test Procedure:
1. Nucleic acid kubviswa:
Commerce RNA yekubvisa kits iripo, magineti bead kudhonza uye spin column kudhirowa kunokurudzirwa kune iyi kit.
2. Kugadzirira Reaction Mix:
● Bvisa iyo 2019-nCoV reaction Sanganisa A/B uye chengeta pakamuri tembiricha kusvika isina kutonhora;
● Tora zvikamu zvinoenderana (Reaction Mix A 16.5μL / T, Reaction Mix B 3.5μL / T) uye sanganisa, uye ipapo aliquot imwe neimwe PCR reaction ne 20μL / tube;
● Wedzera 5μL yeRNA template kana kutonga kusina kunaka kana kutonga kwakanaka, wozovhara chivharo chechubhu;
● Isa iyo reaction tube mu fluorescence PCR chiridzwa, uye isa iyo yakaipa / yakanaka kutonga uye sampuli paramita yeRT-PCR maitiro maererano neiridzwa yekushandisa mirairo.
● Rekodha Sample yekuisa order
3.RT-PCR mitemo:
Recommended Settings:
| Cycle | Nguva | Tembiricha(℃） | |
| 1 | 1 | 10min | 25 |
| 2 | 1 | 10min | 50 |
| 3 | 1 | 10min | 95 |
| 4 | 45 | 10s | 95 |
| 35s | 55 |