Rotavirus/Adenovirus/Norovirus Ag Test
Chinangwa Kushandiswa
Iyo kit inoitirwa kutarisisa uye kwemhando yekuona kweboka A rotavirus antigens, adenovirus antigens 40 uye 41, norovirus (GI) uye norovirus (GII) maantigens mutsvina yevanhu.
Mhedzisiro yebvunzo yakanaka inoda kumwe kusimbiswa.Mhedzisiro isina kunaka yekuongororwa haibvise mukana wekutapukirwa.
Mhedzisiro yebvunzo dzekiti iyi ndeyekuongororwa kwekiriniki chete.Zvinokurudzirwa kuita ongororo yakazara yemamiriro acho zvichienderana nemurwere kuratidzwa kwekiriniki uye mamwe ma laboratory bvunzo.
Summary
Rotavirus (RV)ihosha yakakosha inokonzera hutachiona hwemanyoka uye enteritis mucheche nevana vaduku pasi rose.Iyo yepamusoro yezviitiko iri mumatsutso, inozivikanwawo se "autumn diarrhea yevacheche nevana vadiki".Chiitiko chezvirwere zvehutachiona muvacheche mukati memwedzi uye makore maviri ezera yakakura kusvika ku62%, uye nguva yekudzivirira ndeye 1 kusvika kumazuva manomwe, kazhinji pasi pemaawa makumi mana nemasere, inoratidzwa nekuora kwakanyanya uye kupera simba.Mushure mekupinda mukati memuviri wemunhu, inodzokorora mumaseru akashata epithelial emukati mudumbu uye inoburitswa muhuwandu hukuru netsvina.
Adenovirus (ADV)utachiona hweDNA hwakasungwa kaviri hune dhayamita 70-90nm.Iyo symmetric icosahedral virus isina envelopu.Zvimedu zvehutachiona zvinonyanya kuumbwa nemaprotein shells uye core double stranded DNA.Enteric adenovirus mhando 40 uye mhando 41 yeboka F akakosha utachiona hwemanyoka muvanhu, kunyanya kubata vacheche nevana vadiki (vari pasi pemakore mana).Iyo incubation nguva inenge mazuva matatu kusvika gumi.Inodzokorora mumasero emukati uye inobudiswa mutsvina kwemazuva gumi.Clinical kuratidzwa kurwadziwa mudumbu, manyoka, mvura tsvina, achiperekedzwa fivha uye kurutsa.
Norovirus (NoV)ndeyemhuri yecaliciviridae uye ine 20-hedral particles ine dhayamita ye27-35 nm uye isina hamvuropu.Norovirus ndechimwe chezvirwere zvikuru zvinokonzeresa kwete-bacterial acute gastroenteritis parizvino.Hutachiona uhu hunotapuriranwa zvakanyanya uye hunonyanya kutapuriranwa nemvura ine utachiona, chikafu, kutapurirana utachiona uye aerosol inogadzirwa nezvinosvibisa.Norovirus ndiyo yechipiri huru pathogen inokonzera manyoka ehutachiona muvana, uye inoputika munzvimbo dzakazara.MaNoroviruses anonyanya kukamurwa kuita mashanu genomes (GI, GII, GIII, GIV uye GV), uye hutachiona hwevanhu iGI, GII uye GIV, pakati peiyo GII genome ndiyo inonyanya kufarirwa nehutachiona pasi rese.Makiriniki kana marabhoritari ekuongorora nzira dzehutachiona hwenorovirus dzinonyanya kusanganisira electron microscopy, molecular biology uye immunological diagnosis.
Composition
Kuunganidza uye Kubata sampuli
1. Unganidza sampuli yetsvina isina kurongeka mumudziyo wakachena, wakaoma.
2. Vhura mudziyo wekuunganidza tsvina nekusunungura kumusoro uye shandisa foshoro yekuunganidza kuita zvisina tsarukano
3. baya sampuli yetsvina munzvimbo 2 ~ 5 dzakasiyana kuti uunganidze zvakakomberedza 100mg tsvina yakasimba (yakaenzana ne1/2 yepizi) kana 100μL tsvina yemvura.Usatore sampuli yetsvina nekuti izvi zvinogona kutungamira kune bvunzo isiriyo.
4. Iva nechokwadi chekuti sampuli yetsvina iri mumikoto yefoshoro yekuunganidza.Yakawandisa tsvina yemucherechedzo inogona kutungamira kune isingaite yebvunzo mhinduro.
5. Nyora uye simbisa chivharo pamudziyo wekuunganidza wemuenzaniso.
6. Kuzunungusa mudziyo muunganidzwa wetsvina nesimba.
Test Procedure
1. Uya nemuenzaniso uye zvikamu zvekuedza kune tembiricha yekamuri kana iri mufiriji kana chando.
2. Kana wagadzirira kutanga kuyedza, vhura homwe yakavharwa nekubvarura nepakati.Bvisa bvunzo kubva muhomwe.
3. Isa mudziyo wekuyedza panzvimbo yakachena, yakati sandara.
4. Rongedza mudziyo wekuunganidza tsvina wakatwasuka womonyorora chivharo chemudziyo.
5. Wakabata mudziyo wekuunganidza tsvina wakatarisa mudenga, isa 80μL (inenge madonhwe maviri) emushonga mumuenzaniso wetsime rebvunzo.Usaremedza sampuli.
6. Verenga mhinduro yebvunzo mukati memaminitsi gumi nemashanu.Usaverenge mhedzisiro mushure memaminitsi gumi nemashanu.
Tsanangudzo Yemhinduro
1. Zvakanaka:Kuvepo kwemitsetse miviri tsvuku-yepepuru (T uye C) mukati mehwindo remhedzisiro inoratidza yakanaka yeRV/ADV/NoV antigen.
2. Negative:Mutsetse mumwechete dzvuku-pepuru unoonekwa pamutsetse wekutonga (C) unoratidza mhedzisiro yakaipa.
3. Hazvina basa:Kana mutsara wekutonga (C) ukatadza kuoneka, zvisinei kuti mutsara weT uri kuoneka here kana kuti kwete, bvunzo yacho haina basa.Wongorora maitiro uye dzokorora bvunzo nemudziyo mutsva wekuyedza.
